Practical, and intended for day-to-day use, this state-of-the-art New Edition presents the elements of immunohistochemistry in the context of the "total test". It deals logically with issues of test selection · reagent selection and quantification · protocols and methods · controls · results · interpretation · in situ hybridization · reporting · and significance. In each chapter, readers will find infectious diseases and prognostic markers and a molecular morphology perspective, focusing on DNA, RNA, and protein, thus offering the surgical pathologist a genuine ability to see with molecular eyes. Immunomicroscopy, 3rd Edition is Volume 19 in the Major Problems in Pathology Series. Features an expanded authorship of internationally renowned experts. Offers timely coverage on all diagnostically useful tests and their application and interpretation. Integrates infectious diseases and prognostic markers into each chapter, offering complete diagnostic coverage of any given organ system. Incorporates aspects of molecular morphology throughout. Presents an organ system approach to diagnostic surgical pathology. Includes appendices that address quality assurance and standardization protocols reflecting National Committee for Clinical Laboratory Standards guidelines · tissue microarrays · and laser microscopy and digital immunomicroscopy, including image analysis, spectral imaging, and quantitation. Uses full-color illustrations throughout. With 32 expert contributors.

1 Principles of immunomicroscopy 1 2 Practical issues : fixation, processing and antigen retrieval 47 3 Molecular and special techniques 75 4 Lymphoma including Hodgkin lymphoma 103 5 Adrenal and thyroid disease 155 6 The breast 181 7 The female reproductive tract 209 8 Molecular morphology and immunomicroscopy 225 9 Pediatric diseases 245 10 Soft tissue and bone 261 11 The lung and mesothelium 283 12 The gastrointestinal tract 319 13 Skin tumors, including melanocytic lesions 337 14 The central nervous system 353 15 Tumors of unknown origin 379 16 Cytopathology and fine needle aspiration 397 17 Occult metastases 417