In this third edition of his popular undergraduate-level textbook, Des Nicholl recognises that a sound grasp of basic principles is vital in any introduction to genetic engineering. Therefore, as well as being thoroughly updated, the book also retains its focus on the fundamental principles used in gene manipulation. The text is divided into three sections: Part I provides an introduction to the relevant basic molecular biology; Part II, the methods used to manipulate genes; and Part III, applications of the technology. There is a new chapter devoted to the emerging importance of bioinformatics as a distinct discipline. Other additional features include text boxes, which highlight important aspects of topics discussed, and chapter summaries, which include aims and learning outcomes. These, along with key word listings, concept maps and a glossary, will enable students to tailor their study to suit their own learning styles and ultimately gain a firm grasp of a subject that students traditionally find difficult.

Ch. 1 Introduction 2 Pt. I The basis of genetic engineering Ch. 2 Introducing molecular biology 12 Ch. 3 Working with nucleic acids 32 Ch. 4 The tools of the trade 50 Pt. II The methodology of gene manipulation Ch. 5 Host cells and vectors 62 Ch. 6 Cloning strategies 90 Ch. 7 The polymerase chain reaction 116 Ch. 8 Selection, screening, and analysis of recombinants 132 Ch. 9 Bioinformatics 152 Pt. III Genetic engineering in action Ch. 10 Understanding genes, genomes, and 'otheromes' 170 Ch. 11 Genetic engineering and biotechnology 202 Ch. 12 Medical and forensic applications of gene manipulation 226 Ch. 13 Transgenic plants and animals 256 Ch. 14 The other sort of cloning 278 Ch. 15 Brave new world or genetic nightmare? 291 Suggestions for further reading 297 Using the World Wide Web 299 Glossary 303 Index 327